Development of protocol for the production of Acacia hybrid synthetic seeds
The axillary buds of in vitro Acacia hybrid were cut aseptically where the explants were placed in the encapsulation matrix (sodium alginate solution). The encapsulated explants or synthetic seeds or beads were cultured onto the tissue culture medium and maintained in the culture room at 261°C under a 16 hour photoperiod with a photon flux density of about 35 molm-2s-1 provided by cool white fluorescent lamps to observe the germination ability of the beads.
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